久久在线视频,av中文字幕在线观看,桃色视频在线观看,国产一区二区三精品久久久无广告

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-2279MS1 小鼠胰島內(nèi)皮細(xì)胞

MS1 小鼠胰島內(nèi)皮細(xì)胞

簡(jiǎn)要描述:CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2279
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2103

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞 的詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞

ATCC® Number:  CRL-2279™

Designations:  MS1 (MILE SVEN 1)

Depositors:   JL Arbiser

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped:  froz


Organism: Mus musculus (mouse)en

Medium & Serum:  See Propagation

Growth Properties: adherent

Morphology: endothelial


CRL-2279

Source: Organ: pancreas

Strain: C57BL/6

Tissue: islet of Langerhans; endothelium

Cell Type: SV40 transformed

Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase (high levels)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Receptors: vascular endothelial growth factor (VEGF), expressed

Comments: MS1 is a pancreatic islet endothelial cell line established in 1994.

Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418.

Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.

The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.

The cells are useful for the study of signal transduction of angigenic factors.

It is the only line known that can give rise to benign hemangiomas.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
超碰人妻人人干| 大香蕉热线精品视| 成人精品一区二区三区电影| 欧美日韩一区一区| 免费勾搭国产在线播放| 一本一本久久a久久精品牛牛影视 夜夜躁狠狠躁日日躁2002讲述 | 亚洲AV成人精品一区二区三区| 7777在线| 猛插人妻少妇| 97影院三级片| 精品国产乱码久久久久久久| 久久久一本二本三本| 日本人妻任你躁| 亚洲视频高清无码一区| 海南省| h视频免费观看| 久久国产精品成人影院| 欧美亚洲精选| 你懂的网址亚洲| 久久人人爽人人爽人人片亚洲 | 中文字幕人体大胆艺术| 成人午夜国产内射主播| 国产大屁股视频网站| 久久精品久久精品久久| 伊人22| 欧美国产日韩a欧美在线视频| 无套进入19p| 久久影院国产一区| 亚色网人妻| 蜜芽国产尤物av尤物在线看| 欧美另类日韩| 乳 插 粗 捏 在线| 亚洲韩国日本中文字幕| 日韩综合AV| 久久噜噜| 丁香另类激情自拍| 曰韩一级欧美| 亚洲最大无码| 思思久久破| 91在线视频观看| 国产激情自拍视频|