久久在线视频,av中文字幕在线观看,桃色视频在线观看,国产一区二区三精品久久久无广告

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2234SNU-449細(xì)胞, 人肝癌細(xì)胞

SNU-449細(xì)胞, 人肝癌細(xì)胞

簡要描述:SNU-449細(xì)胞, 人肝癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫管理規(guī)范,提供 *培養(yǎng)條件

  • 產(chǎn)品型號:CRL-2234
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1894

產(chǎn)品分類

Product Category

詳細(xì)介紹

SNU-449細(xì)胞, 人肝癌細(xì)胞(ATCC® CRL-2234)

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissue

liver

Product Formatfrozen
Morphologyepithelial; diffusely spreading cells
Culture Propertiesadherent
Biosafety Level2  [Cells contain Hepatitis B virus]
Diseasegrade II-III/IV,hepatocellular carcinoma
Age52 years
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
Karyotypeaneuploid; modal number = 57
Derivation

SNU-449 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.
Clinical Data

52 years

Asian

male

SNU-449細(xì)胞, 人肝癌細(xì)胞

Comments

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.

Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was predominantly compact and minor trabecular type.

The cultured cells contain a single or double nucleus.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
SubculturingProtocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
天天干天天日干天天射| 91日韩精品一区二区| 揄拍成人国产精品视频| 麻豆av一区| 欧美激情不卡不卡| 国产视频五月开心| 91在线浏览| 亚洲精品国| 亚欧免费无码Aⅴ在线观看| 日韩亚洲欧美一区| 伊人久久首页| 一级黄片黄片一级| 高清欧美亚洲精品| 欧美精产国品一二三| 一个人hd在线观看免费高清视频 | 综合五月欧美| 妻无码一区二区三区| 亚洲77图片| 精品福利导航| 999久久久免费精品国产| 亚洲综合日韩欧美| 中文字幕 无码 另类| 日韩熟女欧美极品| 亚洲日韩精品激情综合| 国内精品伊人久久久久AV一坑| 久久久久久999| 国产熟女精品综合| 伊人开心欧美色| 国产免费久久久无码| 日韩九色| 婷婷在线视频| 激情综合色综合啪啪开心| 国产swag在线| 四虎影院WWWf| 亚洲男人的天堂久久久| 日本色爱视频| 西吉县| 中文乱码字幕高清一区二区| www.色网站| 人人爽日| 欧美新色窝|