久久在线视频,av中文字幕在线观看,桃色视频在线观看,国产一区二区三精品久久久无广告

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11233™THLE-3 細(xì)胞,人肝永生化細(xì)胞

THLE-3 細(xì)胞,人肝永生化細(xì)胞

簡要描述:THLE-3 細(xì)胞,人肝永生化細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:CRL-11233™
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-15
  • 訪  問  量:1729

產(chǎn)品分類

Product Category

詳細(xì)介紹

THLE-3 細(xì)胞,人肝永生化細(xì)胞

traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 0.1% Soybean Trypsin inhibitor  and aspirate cells by gently pipetting.

5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

6.Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new coated culture vessels.

7.Place culture vessels in incubators at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

THLE-3 細(xì)胞,人肝永生化細(xì)胞

Flask Coating

1.Prepare a mixture of 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin (BSA) dissolved in culture medium. Store pre-prepared Coating Solution at 4°C in cold room for up to 3 months.

2.For a growth area of 75 cm2, add 4.5 mL of the fibronectin/collagen/BSA solution and rock gently to coat the entire surface.

3.Incubate the freshly coated vessel(s) in a 37°C incubator overnight (it is preferable to use tissue culture vessels with tightened, plug-seal caps to prevent evaporation during the coating process).

4.Store coated flasks with solution at room temperature, light protected, up to 1 month. Suction off solution before plating cells.


Cryopreservation  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11,12

D13S317: 13

D16S539: 11,12

D5S818: 13

D7S820: 8,10

THO1: 8,9.3

TPOX: 6,9

vWA: 17,18

Name of Depositor  National Cancer Institute

References  Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

References  Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
人妻22P| 日韩一三区| 无码人妻动漫| 全色导航| 欧美婷婷五月天激情| 国产三级在线观看免费| 国产色欲婬乱麻豆天美| 进入成AV网久久国产| 精品高清在线播放| 久久高清免费视频| 亚洲区一区二十大| 久在线精品视频线观看| 日韩一本免费无码视频| 日本全亚洲免费观看| 亚州另类欧美日韩| 久久久久国色ΑV免费观看| www.91干| 97精品视频一区| 色婷婷五月综合欧美| 精品人妻VA出轨中文字幕| 久久久久久免费黄片| 色鬼色综合| 365你懂的| WWW国产亚洲精品| 偷拍视频一区二区| xxxHD麻豆欧美| 五月婷婷之中文自幕| 亚洲精品视觉盛宴无毒| 午夜成人在线视频| 成人区人妻精品一| 肉蒲团夜销魂之性战| 技户晴0121D4| 欧美两个人在线| 国产在线拍偷自揄拍精品| 久操社区| 60熟妇在线观看| 伊人 五月婷婷| 国产欧美日韩ⅤA| 精品深夜AV无码一区二区老年| 久久九九国产精品| 欧美一区二区放荡人妇|